We suggest that serum amylase level should be monitored carefully in patients with copper sulphate poisoning and the possibility of acute pancreatitis must be considered though it may be difficult to confirm it by imaging due to concurrent renal failure. When i did the experiment of enzyme-controlled reaction with starch and amylase, i did two set, one set by adding copper sulphate to the reaction and the other one didn't why the concentration of starch left is higher when i did not use copper sulphate than i use that suppose that copper sulphate is an inhibitor.
Copper sulphate can do a lot of harm to the human body, but very little has been discussed on the effect of copper sulphate on amylase, which is an enzyme that is present in human saliva and in the small intestines, therefore i want to perform an experiment to find out how the concentration of copper sulphate will affect the action of amylase. As mentioned above, heavy metal ions are typical of this type of inhibition copper is a heavy metal ion, so if copper sulphate does inhibit the action of amylase, i can conclude that copper sulphate is a non-competitive irreversible inhibitor.
A copper sulphate is a competitive inhibitor of the enzyme catalase catalase contains an iron haem group located in the center of a porphyrin ring which carries out the reaction adding copper sulphate displaces the iron from the center of the ring, as it has a higher stability constant (k stab. Hypothesis, i predicted that the more copper sulphate added to the solution the longer the reaction would take to complete this was due to the fact that copper sulphate would interfere with the starch for the active sites on the amylase molecules because it is a inhibitor.
Just starting my cw on the effects of copper sulphate on amylase struggling to find scientific knowledge to back up my hypothesis, any ideas i know it is an inhibitor but am reading lots of conflicting info about whether it is competitive/ non-competitive or reversible/irreversible, my understanding is that it is a non-competitive reversible but not 100% sure about this. Investigating the effect of copper sulphate on amylase activity aim the aim of my experiment is to observe the affect on amylase when adding copper sulphate to a starch solution.
My experiment was to find out the effect of changing the copper sulphate concentration on the rate of the hydrolysis of starch to maltose using the enzyme amylase copper sulphate was used because it acted as an inhibitorhypothesis, i predicted that t. This was due to the fact that copper sulphate would meddle with the starch for the brisk sites on the amylase molecules because it is a inhibitor biological knowledge, amylase acts as a gun and starch is a knotty carbohydrate the amylase was used to hydrolyze the tack glycosidic bonds in starch.
What kind of inhibitory effect does copper sulphate have on the reaction between fungal amylase and starch you can a) experiment with different concentrations of copper sulphate to see whether it is acting as an inhibitor.
Copper sulphate was used because it acted as an inhibitor hypothesis, i predicted that the more copper sulphate added to the solution the longer the reaction would take to complete this was due to the fact that copper sulphate would interfere with the starch for the active sites on the amylase molecules because it is a inhibitor.